Description
T4 RNA Ligase 2, truncated (T4 Rnl2 truncated) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of RNA. The enzyme does not require ATP for ligation but does need the pre-adenylated substrate. T4 Rnl2 truncated is expressed from a plasmid in E. coli which encodes the first 249 amino acids of the full length T4 RNA Ligase 2. Unlike the full length ligase, T4 Rnl2 truncated is unable to adenylate the 5´ end of the substrate, and as a result it cannot ligate the phosphorylated 5´ end of RNA or DNA to the 3´ end of RNA. This enzyme, also known as Rnl2 (1-249) has been used for optimized linker ligation for the cloning of microRNAs. This enzyme reduces background ligation because it can only use adenylated primers.
Applications
RNA ligase
Storage
-20°C
Product Components
| Components | 2, 000 U | 10, 000 U |
| T4 RNA Ligase 2, truncated (200, 000 U/mL) | 10 μL | 50 μL |
| 10X T4 RNA Ligase Reaction Buffer | 500 μL | 500 μL |
| 50% PEG 8000 | 500 μL | 1.25 mL |
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