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T4 Gene 32 Protein (10 mg/mL) (RK20546)

Datasheet

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Cat. No.SpecificationPrice
RK20546100 μg₩108,000
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RK20546500 μg₩441,000
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Description

T4 Gene 32 Protein (10 mg/mL), also known as T4 gp32 protein, is a single stranded specific DNA binding protein. The native Gene 32 Protein from bacteriophage T4 (T4gp32) is a single-stranded DNA binding protein that is required for T4 DNA replication, recombination and repair. T4 Gene 32 protein can increase the yield of long PCR products when the reaction contains proteins ranging from 0.5 to 1.0 nmol. The T4 Gene 32 protein can increase the production of PCR products，reducing the inhibitor influence of humic acid. Meanwhile, the protein can promote the digestion reaction of restriction endonucleases, reverse transcription efficiency in RT-PCR, and enhance the activity of T4 DNA polymerase.
Applications
PCR optimization
Stimulate in vitro DNA synthesis
Stability of DNA or RNA single stranded regions
Site specific mutation experiment
Help limit enzyme digestion completion
q-PCR
Recombinase polymerase amplification, RPA
Storage
Upon receipt, store all components at -20°C

Product Information

Product Components
ComponentsComponent numberSize-1
100 μg		Size-2
500 μg
T4 Gene 32 Protein (10 mg/mL)RM2053210 μL50 μL
Product Data
1. Increase reverse transcription efficiency

M stands for DNA marker, 100bp to 5000 bp
Lane 1 to Lane 4 Reverse transcription substrate: 1ug of RNA; The final concentration of R20546 T4 Gene 32 Protein is 0, 50, 100, 200 ng/uL;
Lane 5 to Lane 8: Reverse transcription substrate:0.5ug RNA; The final concentration of R20546 T4 Gene 32 Proteinis 0, 50, 100, 200 ng/uL;
Conclusion: By comparing the most bright band, user can tell improved RT efficiency based on same RNA input. R20546 T4 Gene 32 Protein can promote reverse transcription efficiency in RT-PCR.

2. Improve RPA specificity

M stands for DNA marker；
Lane 1: Blank control; Lanes 2 to Lane 3: RK20546; Lane 4: Competitor N*; Lane 5: Competitor Y*;
Conclusion: T4 gene32 protein significantly increased the yield and specificity of RPA products.

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