Description
Taq DNA polymerase M101 has 5'→3' polymerase activity and 5'→3' exonuclease activity, but no 3'→5' exonuclease activity. The PCR product has A-tailing at the 3' end, which can be cloned with dT/dU vector, and is suitable for downstream PCR, qPCR, RT-qPCR and NGS application.
Product Features
- Good thermal stability, can be stored for 7 days at 37℃ and not affect product performance.
- Rigorous quality control, lots-to-lots consistency.
Storage
Upon receipt, store all components at -20°C.
Product Components
| Components | 250U | 1, 000U |
| Taq DNA Polymerase M101 (5, 000 U/mL) | 50 μL | 200 μL |
| 10X PCR Reaction Buffer, Mg2+ plus | 1.25 mL | 1.25 mL |
Product Data
1. Excellent amplification efficiency and linearity
| Template Concentration( /μL) | 10 ng | 1 ng | 100 pg | 10 pg | 1 pg | 0.1pg | E（%） | R2 |
| Ct | 12.429 | 15.418 | 19.060 | 22.435 | 25.671 | 29.439 | 96.6 | 0.9992 |
- Use gDNA as the template, after gradient dilution , then input 2 μL diluted template for amplification. The results showed that Taq DNA Polymerase M101 had good amplification linearity and amplification efficiency.
2. Robust Stability
- Use gDNA as the template, and conduct the thermal stability test at 37℃. The results showed that Taq DNA Polymerase M101 had stable performance if placed at 37℃ for 7 days.
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