ABclonal First Strand Synthesis Module (Stranded) (RK20353) is designed for RNA fragmentation and first strand cDNA synthesis in RNA-seq library construction. The second strand cDNA can be directly synthesized without purifying the first strand cDNA product. The RT Reagent in this module does not contain Actinomycin D and can be used with ABclonal Second Strand Synthesis Module (RK20346) to make RNA libraries. In addition, Frag/Elute Buffer employs Mg2+ at high temperature conditions to fragment the RNA. Therefore, the RNA sample cannot contain metal ions such as Mg2+ or a metal ion chelating agent such as EDTA, which may affect the fragmentation size of RNA.
An integrated multi-omics approach revealed the regulation of melatonin on age-dependent mitochondrial function impair and lipid dyshomeostasis in mice hippocampus
Effect of supplementing lysins and methionine to low-protein diets on growth performance, hepatic antioxidant capacity, immune status, and glycolytic activity of tibetan sheep - PMC
Jiajiejian gel ameliorates thyroid nodules through regulation of thyroid hormones and suppression of the (IL-6, TNF-, IL-1)/JAK2/STAT3/VEGF pathway - PMC
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