Description
The DNA Frag Module has been optimized to convert intact DNA to fragmented DNA by generating dsDNA breaks in a time-dependent manner to yield fragments of desired size. The fragmented DNA product is suitable for subsequent DNA library construction. This kit has broad compatibility with DNA samples from different species. For formalin-fixed paraffin-embedded (FFPE) samples, this kit demonstrates excellent performance and library yield. This module contains DNA Frag Reaction Buffer, DNA Frag Enzyme Mix with 1X TE Buffer Reaction Supplement, and 500 mM EDTA.
Product Features
- Easy to operate and shorten the experiment time;
- Flexible combination with ABclonal or other brand kits;
- Compatible with TE and H2O reaction systems;
- Half system can be used;
Workflow Chart
Storage
All components should be stored at -20°C.
The shelf lives of all reagents are one year when stored properly.
Product Components
| Components | 8 RXN | 24 RXN | 96 RXN |
| DNA Frag Reaction Buffer | 32 μL | 96 μL | 384 μL |
| DNA Frag Enzyme Mix | 32 μL | 96 μL | 384 μL |
| 1X TE Buffer | 320 μL | 960 μL | 3840 μL |
| 500 mM EDTA | 40 μL | 120 μL | 480 μL |
Product Data
1. High library yield
200 ng FFPE gDNA was used as a template for ultrasound interruption and DNA Frag Module (RK20260) fragmentation. The Rapid Plus DNA Lib Prep Kit for Illumina V2 (RK20255) was used to construct the library, and the yield of enzymatic fragmentation was higher than that of ultrasonic interruption。
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