The FS Pro DNA Lib Prep Kit for Illumina is designed for DNA library preparation for next generation sequencing (NGS) on Illumina® platforms. This kit integrates DNA fragmentation with end repair and dA tailing into one step, and it can be directly followed by adapter ligation without purification. With greatly simplified experimental flow, the entire experiment can be completed within 2 hours. This kit is compatible with DNA samples from different species and different origins. Optimization of reagents and protocol greatly reduced rates of false positives, significantly improving sequencing yield and overall results, including with FFPE DNA samples. This kit is suitable for the construction of PCR-Free libraries (e.g., 100 ng of high-quality genomic DNA).
Product Features
Perform fragmentation, end repair and dA-tailing with a single enzyme mix；
Entire experiment completed within 2 hours.;
Generate a wide range of insert sizes using vary fragmention time;
Fewer inversions and lower chimeras;
Workflow Chart
Storage
All components should be stored at -20°C. The shelf lives of all reagents are one year when stored properly.
Product Information
Product Components
Components
8 RXN
24 RXN
96 RXN
Fragmentation, End Repair & dA Tailing
FS Pro Buffer I
40 μL
120 μL
480 μL
FS Pro Enzymes I
80 μL
240 μL
960 μL
1X TE Buffer
280 μL
840 μL
1.68 mL
Adapter Ligation
FS Pro Ligation Buffer I
160 μL
480 μL
1.92 mL
Ligation Enzymes
40 μL
240 μL
960 μL
Amplification
Gloria Nova HS 2X PCR Mix for NGS
200 μL
600 μL
2.4 mL
10X PCR Primers
40 μL
120 μL
480 μL
Product Data
1. Generate a wide range of insert sizes using vary fragmention time.
100 ng human blood gDNA initial template was treated with this kit. Fragmentation was performed 32°C for 5/10/15/20 /25/30 min.
2. Lower false positives
100 ng FFPE DNA was starting template for library construction using this kit and other kits. Compared with other kits, FS Pro DNA Lib Prep Kit for Illumina has fewer inversions and lower chimeras. This indicates that the library construction using FS kit has less impact on subsequent analysis, and the results are more realistic.
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Genetic Signatures from Adaptation of Bacteria to Lytic Phage Identify Potential Agents to Aid Phage-Killing of Multidrug-Resistant Acinetobacter baumannii
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