제품 > NGS 라이브러리 준비

Fast Magnetic DNA Methylation Bisulfite Kit (RK30206)

Datasheet

Select size and quantity
Cat. No.SpecificationPrice
RK3020618 RXN₩180,800
Add to Cart
RK3020650 RXN₩448,200
Add to Cart

Description

This kit quickly converts unmethylated cytosines in DNA samples to uracil while leaving methylated cytosines unchanged. After bisulfite treatment at high temperature, double-stranded DNA is denatured into single strands. Under the action of HSO3-, cytosine residues undergo deamination and are converted to uracil, while methylated residues remain intact. Subsequently, during PCR amplification, uracil is replaced by thymine (T).
Storage

Store Conversion Reagent at room temperature (15 ~ 25℃) away from light. Other components should also be stored at room temperature (15 ~ 25℃).

Product Information

Product Components

Components NameComponents NumberSize-1
18 RXN
Size-2
50 RXN
Conversion ReagentRM305141.7 mL × 21.5 mL × 7
Clean BeadsRM30515300 μL850 μL
Wash BufferRM305165 mL14 mL
Desulphonation BufferRM305174 mL11 mL
Elution BufferRM305181 mL1.8 mL
Product Data

1. Conversion Efficiency

Using the ABclonal Scale Methyl-DNA Lib Prep Kit for Illumina (Cat.No.RK20220) in conjunction with the Fast Magnetic DNA Methylation Bisulfite Kit (Cat.No.RK30206) for parallel library construction tests with the Vendor's transformation kit, the input amounts were set at 10 ng and 100 ng (the sample was 293F gDNA mixed with 1% lambda DNA and 0.1% Puc19). The results showed that the performance of RK30206 and the Vendor's transformation kit in the conversion efficiency of unmethylated cytosines was consistent.

2. Sequencing Quality

Using the ABclonal Scale Methyl-DNA Lib Prep Kit for Illumina (Cat.No.RK20220) (Catalog No. RK20220) in combination with the Fast Magnetic DNA Methylation Bisulfite Kit (Catalog No. RK30206) for parallel library construction testing with the Vendor's transformation kit, the input amounts were set at 10 ng and 100 ng (the sample was 293F gDNA mixed with 1% lambda DNA and 0.1% Puc19). The results indicated that the RK30206 and the Vendor's transformation kit performed equally in terms of sequencing data quality.

Documents

Products Categories
Contact us to order