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mRNA-seq Lib Prep Module for Illumina (RK20350)

Datasheet

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Cat. No.SpecificationPrice
RK2035024 RXN₩1,183,500
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RK2035096 RXN₩4,017,000
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RK203508 RXN₩472,500
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Description

The mRNA-seq Lib Prep Module for Illumina® kits provides a method for generating non-directional (non-strand-specific) RNA library that are compatible with the Illumina sequencing platform. The kit contains all of the enzymes and buffers for the First Strand Synthesis Module, Second Synthesis Module, and DNA Lib Prep Module. Its Compatible with Poly(A) mRNA Purification Module(RK20341), rRNA Depletion module (H/M/R)(RK20348)and multiplexing adaptors and primers. The kit is applicable to animal, plant, and eukaryote (e.g., fungi) total RNA templates as well as the isolated and purified mRNA templates. The recommended total RNA input is 100 ng-1 μg.
Workflow Chart

Storage

-20℃

Product Information

Product Components

Tube Name24 rxns96 rxns
2X Frag/Elute Buffer 144 μL 576 μL
RT Reagent 192 μL 768 μL
First Strand Synthesis Enzyme Mix 48 μL 192 μL
Second Strand Synthesis Reaction Buffer 192 μL 768 μL
Second Strand Synthesis Enzyme Mix 96 μL 384 μL
Nuclease-free Water 1 ml X 2 8 mL
End-prep Buffer 240 μL 960 μL
End-prep Enzyme Mix 72 μL 288 μL
Ligation Buffer 396 μL 1584 μL
Ligase Mix 72 μL 288 μL
2X PCR Mix 600 μL 1200 μl X 2
2X PCR Mix 1 mL X 3 10 mL

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