The Rapid DNA Lib Prep kit provides a streamlined and efficient method to construct DNA library for next generation sequencing (NGS) with Illumina (R) platforms. The entire protocol can be completed in 2 hours. Starting from fragmented input-DNA (1 - 1000 ng), the kit contains all the enzymes and buffers for end-preparation, adapter ligation and library amplification. To simplify the workflow, the fragmented DNA is end-repaired and adenylated during the initial step. The kit can be conveniently used for construction of PCR-free libraries from 100 ng of high-quality fragmented genomic DNA, or 10 ng of circulating cell-free DNA (cfDNA). PCR primers provided in the kit are optional for users who prefer self-selected high-fidelity DNA polymerases in the library amplification.
Product Features
Input DNA amount: 1ng-1ug High DNA library conversion, and less PCR amplification cycles; Suitable for FFPE DNA and cfDNA Reduced PCR bias Lower cost per library
Workflow Chart
Storage
The Low-EDTA TE buffer can be stored at room temperature or 4°C. All other components should be stored at -20°C. The shelf lives of all reagents are one year when stored properly.
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