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4-HNE Rabbit pAb (A24456)

Publications (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - 4-HNE Rabbit pAb (A24456)

Western blot analysis of lysates from HeLa cells, using 4-HNE Rabbit pAb (A24456) at 1:1000 dilution. HeLa treated with 4-Hydroxynonenal (4-HNE).
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

Overview

Product name4-HNE Rabbit pAb
Catalog No.A24456
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenChemical compounds corresponding to 4-HNE.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW42-350kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa treated with 4-Hydroxynonenal
Cellular location
Customer validation

WB(Homo sapiens)

IF(Mus musculus)

Other(Homo sapiens)

IHC(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - 4-HNE Rabbit pAb (A24456)}

    Western blot - 4-HNE Rabbit pAb (A24456)

    Western blot analysis of lysates from HeLa cells, using 4-HNE Rabbit pAb (A24456) at 1:1000 dilution. HeLa treated with 4-Hydroxynonenal (4-HNE).
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 20s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A24456? Please let us know so that we can cite the reference in this datasheet.