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4-Hydroxynonenal Rabbit mAb (A26085)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - 4-Hydroxynonenal Rabbit mAb (A26085)

Western blot analysis of lysates from HeLa cells using 4-Hydroxynonenal Rabbit mAb (A26085) at 1:50000 dilution incubated overnight at 4℃. HeLa cells were treated by 4-HNE (0.2 mg/ml) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - 4-Hydroxynonenal Rabbit mAb (A26085)

Confocal imaging of HeLa cells (treated with 4-HNE) and HeLa cells (untreated) using 4-Hydroxynonenal Rabbit mAb (A26085, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Flow CytoMetry - 4-Hydroxynonenal Rabbit mAb (A26085)

Flow cytometry: 1X10^6 HeLa cells (negative control, left) and HeLa cells (treated with 4-Hydroxynonenal, right) were intracellularly-stained with 4-Hydroxynonenal Rabbit mAb (A26085, 2 μg/mL, orange line) or Rabbit IgG isotype control (AC042, 2 μg/mL, blue line), followed by FITC conjugated goat anti-Rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - 4-Hydroxynonenal Rabbit mAb (A26085)

Flow cytometry: 1X10^6 HeLa cells (treated with 4-Hydroxynonenal) were intracellularly stained with Rabbit IgG isotype control (AC042, 2 μg/mL, left) or 4-Hydroxynonenal Rabbit mAb (A26085, 2 μg/mL, right), followed by FITC conjugated goat anti-Rabbit pAb staining.

Overview

Product name4-Hydroxynonenal Rabbit mAb
Catalog No.A26085
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC70314
4-hydroxy-2-nonenal (4-hydroxynonenal, 4-HNE) is a highly reactive aldehyde generated by the exposure of polyunsaturated fatty acids to peroxides and reactive oxygen species (ROS). It non-enzymatically forms stable protein adducts with histidine, lysine, and cysteine side chains that have been used as biomarkers for oxidative damage in cells. Conditions where 4-HNE immunoreactivity has been observed include include inflammation, neurodegenerative diseases, and ischemic damage to the heart and brain.
ImmunogenChemical compounds corresponding to 4-Hydroxynonenal.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms4-HNE
Calculated MW
Observed MW23-310kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:10000 - 1:50000
  • IF/ICC 1:200 - 1:500
  • FC (intra) 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa cells treated with 4-Hydroxynonenal
Cellular locationCell membrane, Cytoplasm, Nucleus.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - 4-Hydroxynonenal Rabbit mAb (A26085)}

    Western blot - 4-Hydroxynonenal Rabbit mAb (A26085)

    Western blot analysis of lysates from HeLa cells using 4-Hydroxynonenal Rabbit mAb (A26085) at 1:50000 dilution incubated overnight at 4℃. HeLa cells were treated by 4-HNE (0.2 mg/ml) at 37℃ for 30 minutes.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 30 μg per lane.
    Blocking buffer: 3 % nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunofluorescence - 4-Hydroxynonenal Rabbit mAb (A26085)}

    Immunofluorescence - 4-Hydroxynonenal Rabbit mAb (A26085)

    Confocal imaging of HeLa cells (treated with 4-HNE) and HeLa cells (untreated) using 4-Hydroxynonenal Rabbit mAb (A26085, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Flow CytoMetry - 4-Hydroxynonenal Rabbit mAb (A26085)}

    Flow CytoMetry - 4-Hydroxynonenal Rabbit mAb (A26085)

    Flow cytometry: 1X10^6 HeLa cells (negative control, left) and HeLa cells (treated with 4-Hydroxynonenal, right) were intracellularly-stained with 4-Hydroxynonenal Rabbit mAb (A26085, 2 μg/mL, orange line) or Rabbit IgG isotype control (AC042, 2 μg/mL, blue line), followed by FITC conjugated goat anti-Rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - 4-Hydroxynonenal Rabbit mAb (A26085)}

    Flow CytoMetry - 4-Hydroxynonenal Rabbit mAb (A26085)

    Flow cytometry: 1X10^6 HeLa cells (treated with 4-Hydroxynonenal) were intracellularly stained with Rabbit IgG isotype control (AC042, 2 μg/mL, left) or 4-Hydroxynonenal Rabbit mAb (A26085, 2 μg/mL, right), followed by FITC conjugated goat anti-Rabbit pAb staining.

    * For research use only. Not for therapeutic or diagnostic purposes.