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5-Hydroxymethylcytosine/ 5-hmC Rabbit pAb (A20140)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Dot Blot - 5-Hydroxymethylcytosine/ 5-hmC Rabbit pAb (A20140)

Dot-blot analysis of 5-Hydroxymethylcytosine(5-hmC), 5-Methylcytosine (5mC) and unmodified adenosine using 5-Hydroxymethylcytosine/ 5-hmC antibody (A20140) at 1:500/1:2000 dilution.

m5C 1 - 5’CAGTAACTGTGGTCCGGTAACTGACTTGCA3’

m5C 2 - 5’CAGTAACTGTGGTC/i5MedC/GGTAACTGACTTGCA3’

Overview

Product name5-Hydroxymethylcytosine/ 5-hmC Rabbit pAb
Catalog No.A20140
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
In mammalian genomes, 5-mC can be enzymatically oxidized to 5-hmC (5-hydroxymethylcytosine). This modification is suggested to be an intermediate between methylation and demethylation of the genome. Occupancy of 5-hmC correlates with inactive or non-productive promoters (PMID: 21925312). 5-hmC pattern is different from 5-methylcytosine (5-mC) in many instances(PMID: 21610077).
ImmunogenChemical compounds corresponding to 5-hydroxymethylcytidine / hm5C.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms5-hmC; 5hmc; 5-HydroxyMethyl cytidine; hm5c; 5-Hydroxymethylcytosine/ 5-hmC
Calculated MW
Observed MWRefer to figures
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • DB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application
Positive samples
Cellular location

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Dot Blot - 5-Hydroxymethylcytosine/ 5-hmC Rabbit pAb (A20140)}

    Dot Blot - 5-Hydroxymethylcytosine/ 5-hmC Rabbit pAb (A20140)

    Dot-blot analysis of 5-Hydroxymethylcytosine(5-hmC), 5-Methylcytosine (5mC) and unmodified adenosine using 5-Hydroxymethylcytosine/ 5-hmC antibody (A20140) at 1:500/1:2000 dilution.

    m5C 1 - 5’CAGTAACTGTGGTCCGGTAACTGACTTGCA3’

    m5C 2 - 5’CAGTAACTGTGGTC/i5MedC/GGTAACTGACTTGCA3’

    * For research use only. Not for therapeutic or diagnostic purposes.