제품 > 항체 > Other Modified Antibodies

5-Methylcytosine (5mC) Rabbit mAb (A20599)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Dot Blot - 5-Methylcytosine (5mC) Rabbit mAb (A20599)

Dot-blot analysis of 5-Methylcytosine (5mC) and unmodified cytosine using 5-Methylcytosine (5mC) Rabbit mAb antibody (A20599) at 1:1000 dilution.
5-Methylcytosine (5mC) : Biotin-5'CGATAACCACTAGT(5mC)3'
unmodified cytosine : Biotin-5'CGATAACCACTAGTC3'

Overview

Product name5-Methylcytosine (5mC) Rabbit mAb
Catalog No.A20599
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC50801
In the mammalian genome, DNA methylation is an epigenetic mechanism involving the transfer of a methyl group onto the C5 position of the cytosine to form 5-methylcytosine. DNA methylation regulates gene expression by recruiting proteins involved in gene repression or by inhibiting the binding of transcription factor(s) to DNA. During development, the pattern of DNA methylation in the genome changes as a result of a dynamic process involving both de novo DNA methylation and demethylation. As a consequence, differentiated cells develop a stable and unique DNA methylation pattern that regulates tissue-specific gene transcription.? Intriguingly, postmitotic neurons still express DNA methyltransferases and components involved in DNA demethylation. Moreover, neuronal activity can modulate their pattern of DNA methylation in response to physiological and environmental stimuli. The precise regulation of DNA methylation is essential for normal cognitive function.
Immunogen5mC
Sequence5mC conjugated to KLH
Gene ID
Swiss Prot
Synonyms5mC; 5-Methylcytosine (5mC)
Calculated MW
Observed MW
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • DB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application
Positive samples5-Methylcytosine (5mC)
Cellular location
Customer validation

DB(C. albicans, Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Dot Blot - 5-Methylcytosine (5mC) Rabbit mAb (A20599)}

    Dot Blot - 5-Methylcytosine (5mC) Rabbit mAb (A20599)

    Dot-blot analysis of 5-Methylcytosine (5mC) and unmodified cytosine using 5-Methylcytosine (5mC) Rabbit mAb antibody (A20599) at 1:1000 dilution.
    5-Methylcytosine (5mC) : Biotin-5'CGATAACCACTAGT(5mC)3'
    unmodified cytosine : Biotin-5'CGATAACCACTAGTC3'

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A20599? Please let us know so that we can cite the reference in this datasheet.