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ABflo® 450 Mouse IgG 2a isotype control (A25417)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Flow CytoMetry - ABflo® 450 Mouse IgG 2a isotype control (A25417)

Flow cytometry: 1X10^6 Raji cells (negative control, left) and Human PBMC (right) were surface-stained with ABflo® 450 Mouse anti-Human CD3 mAb (A25189, 5 μl/Test, orange line) or ABflo® 450 Mouse IgG 2a isotype control (A25417, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Overview

Product nameABflo® 450 Mouse IgG 2a isotype control
Catalog No.A25417
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG
CloneNo.AMC50006-ABf450
Immunogen
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular location
    ABclonal:Flow CytoMetry - ABflo® 450 Mouse IgG 2a isotype control (A25417)}

    Flow CytoMetry - ABflo® 450 Mouse IgG 2a isotype control (A25417)

    Flow cytometry: 1X10^6 Raji cells (negative control, left) and Human PBMC (right) were surface-stained with ABflo® 450 Mouse anti-Human CD3 mAb (A25189, 5 μl/Test, orange line) or ABflo® 450 Mouse IgG 2a isotype control (A25417, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

    * For research use only. Not for therapeutic or diagnostic purposes.