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ABflo® 450 Rabbit IgG isotype control (A27451)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Flow CytoMetry - ABflo® 450 Rabbit IgG isotype control (A27451)

Flow cytometry: 1X10^6 Human PBMC were surface-stained with ABflo® 450 Rabbit anti-Human CD4 mAb (A26523, 5 μl/Test, orange line) or ABflo® 450 Rabbit IgG isotype control (A27451, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 450 Rabbit IgG isotype control (A27451)

Flow cytometry: 1X10^6 Human PBMC were surface-stained with ABflo® 450 Rabbit IgG isotype control (A27451, 5 μl/Test, left) or ABflo® 450 Rabbit anti-Human CD4 mAb (A26523, 5 μl/Test, right).

ABclonal:Flow CytoMetry - ABflo® 450 Rabbit IgG isotype control (A27451)

Flow cytometry: 1X10^6 HeLa cells (negative control, left) and THP-1 cells (right) were surface-stained with ABflo® 450 Rabbit anti-Human CD4 mAb (A26523, 5 μl/Test, orange line) or ABflo® 450 Rabbit IgG isotype control (A27451, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 450 Rabbit IgG isotype control (A27451)

Flow cytometry: 1X10^6 THP-1 cells were surface-stained with ABflo® 450 Rabbit IgG isotype control (A27451, 5 μl/Test, left) or ABflo® 450 Rabbit anti-Human CD4 mAb (A26523, 5 μl/Test, right).

Overview

Product nameABflo® 450 Rabbit IgG isotype control
Catalog No.A27451
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC5105-10-ABflo450
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen.An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
ImmunogenA synthesized peptide derived from rabbit IgG isotype control.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular location
    ABclonal:Flow CytoMetry - ABflo® 450 Rabbit IgG isotype control (A27451)}

    Flow CytoMetry - ABflo® 450 Rabbit IgG isotype control (A27451)

    Flow cytometry: 1X10^6 Human PBMC were surface-stained with ABflo® 450 Rabbit anti-Human CD4 mAb (A26523, 5 μl/Test, orange line) or ABflo® 450 Rabbit IgG isotype control (A27451, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 450 Rabbit IgG isotype control (A27451)}

    Flow CytoMetry - ABflo® 450 Rabbit IgG isotype control (A27451)

    Flow cytometry: 1X10^6 Human PBMC were surface-stained with ABflo® 450 Rabbit IgG isotype control (A27451, 5 μl/Test, left) or ABflo® 450 Rabbit anti-Human CD4 mAb (A26523, 5 μl/Test, right).
    ABclonal:Flow CytoMetry - ABflo® 450 Rabbit IgG isotype control (A27451)}

    Flow CytoMetry - ABflo® 450 Rabbit IgG isotype control (A27451)

    Flow cytometry: 1X10^6 HeLa cells (negative control, left) and THP-1 cells (right) were surface-stained with ABflo® 450 Rabbit anti-Human CD4 mAb (A26523, 5 μl/Test, orange line) or ABflo® 450 Rabbit IgG isotype control (A27451, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 450 Rabbit IgG isotype control (A27451)}

    Flow CytoMetry - ABflo® 450 Rabbit IgG isotype control (A27451)

    Flow cytometry: 1X10^6 THP-1 cells were surface-stained with ABflo® 450 Rabbit IgG isotype control (A27451, 5 μl/Test, left) or ABflo® 450 Rabbit anti-Human CD4 mAb (A26523, 5 μl/Test, right).

    * For research use only. Not for therapeutic or diagnostic purposes.