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ABflo® 488 Rabbit anti-4-Hydroxynonenal mAb (A26080)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-4-Hydroxynonenal mAb (A26080)

Flow cytometry: 1X10^6 HeLa cells (negative control, left) and HeLa cells (treated with 4-Hydroxynonenal, right) were intracellularly-stained with ABflo® 488 Rabbit anti-4-Hydroxynonenal mAb (A26080, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-4-Hydroxynonenal mAb (A26080)

Flow cytometry: 1X10^6 HeLa cells (treated with 4-Hydroxynonenal) were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-4-Hydroxynonenal mAb (A26080, 5 μl/Test, right).

Overview

Product nameABflo® 488 Rabbit anti-4-Hydroxynonenal mAb
Catalog No.A26080
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC70314-ABflo488
4-hydroxy-2-nonenal (4-hydroxynonenal, 4-HNE) is a highly reactive aldehyde generated by the exposure of polyunsaturated fatty acids to peroxides and reactive oxygen species (ROS). It non-enzymatically forms stable protein adducts with histidine, lysine, and cysteine side chains that have been used as biomarkers for oxidative damage in cells. Conditions where 4-HNE immunoreactivity has been observed include include inflammation, neurodegenerative diseases, and ischemic damage to the heart and brain.
ImmunogenChemical compounds corresponding to 4-Hydroxynonenal.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms4-HNE
Calculated MW
Observed MW
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC (intra) 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application
Positive samples
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-4-Hydroxynonenal mAb (A26080)}

    Flow CytoMetry - ABflo® 488 Rabbit anti-4-Hydroxynonenal mAb (A26080)

    Flow cytometry: 1X10^6 HeLa cells (negative control, left) and HeLa cells (treated with 4-Hydroxynonenal, right) were intracellularly-stained with ABflo® 488 Rabbit anti-4-Hydroxynonenal mAb (A26080, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-4-Hydroxynonenal mAb (A26080)}

    Flow CytoMetry - ABflo® 488 Rabbit anti-4-Hydroxynonenal mAb (A26080)

    Flow cytometry: 1X10^6 HeLa cells (treated with 4-Hydroxynonenal) were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-4-Hydroxynonenal mAb (A26080, 5 μl/Test, right).

    * For research use only. Not for therapeutic or diagnostic purposes.