제품 > 항체 > 단일클론항체(mAb)

ABflo® 488 anti-Mouse CD80 mAb (A26801)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Flow CytoMetry - ABflo® 488 anti-Mouse CD80 mAb (A26801)

Flow cytometry: 1X10^6 P815 cells (negative control, left) and C2C12 cells (right) were surface-stained with ABflo® 488 anti-Mouse CD80 mAb (A26801, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 488 anti-Mouse CD80 mAb (A26801)

Flow cytometry: 1X10^6 C2C12 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 anti-Mouse CD80 mAb (A26801, 5 μl/Test, right).

Overview

Product nameABflo® 488 anti-Mouse CD80 mAb
Catalog No.A26801
Host speciesArmenian Hamster
Purification methodAffinity purification
IsotypeIgG
CloneNo.ACC0012-ABf488
Predicted to enable coreceptor activity. Acts upstream of or within T cell costimulation; cellular response to lipopolysaccharide; and positive regulation of alpha-beta T cell proliferation. Located in external side of plasma membrane. Is expressed in heart ventricle and skin. Human ortholog(s) of this gene implicated in type 1 diabetes mellitus. Orthologous to human CD80 (CD80 molecule).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 38-245 of Mouse CD80.
SequenceVDEQLSKSVKDKVLLPCRYNSPHEDESEDRIYWQKHDKVVLSVIAGKLKVWPEYKNRTLYDNTTYSLIILGLVLSDRGTYSCVVQKKERGTYEVKHLALVKLSIKADFSTPNITESGNPSADTKRITCFASGGFPKPRFSWLENGRELPGINTTISQDPESELYTISSQLDFNTTRNHTIKCLIKYGDAHVSEDFTWEKPPEDPPDSK
Gene ID
Swiss Prot
SynonymsB71; Ly53; TSA1; Cd28l; Ly-53; MIC17
Calculated MW34kDa
Observed MW
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationMembrane, Single-pass type I membrane protein.
    ABclonal:Flow CytoMetry - ABflo® 488 anti-Mouse CD80 mAb (A26801)}

    Flow CytoMetry - ABflo® 488 anti-Mouse CD80 mAb (A26801)

    Flow cytometry: 1X10^6 P815 cells (negative control, left) and C2C12 cells (right) were surface-stained with ABflo® 488 anti-Mouse CD80 mAb (A26801, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 488 anti-Mouse CD80 mAb (A26801)}

    Flow CytoMetry - ABflo® 488 anti-Mouse CD80 mAb (A26801)

    Flow cytometry: 1X10^6 C2C12 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 anti-Mouse CD80 mAb (A26801, 5 μl/Test, right).

    * For research use only. Not for therapeutic or diagnostic purposes.