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ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L) (AS035)

Publications (10) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Immunofluorescence - ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L) (AS035)

Immunofluorescence analysis of HeLa cells, using PARP1 antibody (A0942) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C.
Secondary antibody: ABflo® 488-conjugated Donkey Anti-Rabbit IgG (H+L) (AS035) at 1:100 dilution.
Blue: DAPI for nuclear staining.

ABclonal:Flow CytoMetry - ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L) (AS035)

Flow cytometry:1X10^6 K-562 cells (negative control, left) and A-431 cells (right) were surface-stained with Purified Rabbit anti-Human E-Cadherin mAb(5 μl/Test, orange line) or secondary antibody only (blue line).Non-fluorescently stained K-562 and A-431 cells were used as blank control (red line). ABflo® 488-conjugated Donkey Anti-Rabbit IgG (H+L)(AS035, 1:800）was used as a secondary antibody.

Overview

Product nameABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
Catalog No.AS035
Host speciesDonkey
Purification methodAffinity purification
IsotypeABflo™ 488 conjugated IgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenRabbit IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IF/ICC 1:100 - 1:800
  • FC 1:100 - 1:800
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.025% Sodium Azide, 0.75% BSA, 50% glycerol, pH7.3.
Key applicationImmunofluorescence    Flow Cytometry    
Positive samples
Cellular location
Customer validation

IF(Oryza sativa, Homo sapiens, Rattus norvegicus, Mus musculus)

WB(Mus musculus)

FC(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Immunofluorescence - ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L) (AS035)}

    Immunofluorescence - ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L) (AS035)

    Immunofluorescence analysis of HeLa cells, using PARP1 antibody (A0942) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C.
    Secondary antibody: ABflo® 488-conjugated Donkey Anti-Rabbit IgG (H+L) (AS035) at 1:100 dilution.
    Blue: DAPI for nuclear staining.
    ABclonal:Flow CytoMetry - ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L) (AS035)}

    Flow CytoMetry - ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L) (AS035)

    Flow cytometry:1X10^6 K-562 cells (negative control, left) and A-431 cells (right) were surface-stained with Purified Rabbit anti-Human E-Cadherin mAb(5 μl/Test, orange line) or secondary antibody only (blue line).Non-fluorescently stained K-562 and A-431 cells were used as blank control (red line). ABflo® 488-conjugated Donkey Anti-Rabbit IgG (H+L)(AS035, 1:800）was used as a secondary antibody.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS035? Please let us know so that we can cite the reference in this datasheet.