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ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L) (AS073)

Publications (6) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:-

ABclonal:Immunofluorescence - ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L) (AS073)

Immunofluorescence analysis of HeLa cells using Alpha-tubulin (ubiquitous) chain Rabbit mAb (AC039) (40x lens), the secondary antibody was ABflo® 488-conjugated AffiniPure Goat Anti antibody (AS073) used at dilution of 1 : 200. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L) (AS073)

Immunofluorescence analysis of NIH/3T3 cells using Alpha-tubulin (ubiquitous) chain Rabbit mAb (AC039) (40x lens), the secondary antibody was ABflo® 488-conjugated AffiniPure Goat Anti antibody (AS073) used at dilution of 1 : 200. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L) (AS073)

Immunofluorescence analysis of PC-12 cells using Alpha-tubulin (ubiquitous) chain Rabbit mAb (AC039) (40x lens), the secondary antibody was ABflo® 488-conjugated AffiniPure Goat Anti antibody (AS073) used at dilution of 1 : 200. Blue: DAPI for nuclear staining.

ABclonal:Flow CytoMetry - ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L) (AS073)

Flow cytometric analysis of Positive antibody E-Cadherin Rabbit mAb (2.5μg/mL) in various cells (orange) compare to Rabbit rabbit isotype control (blue) and non-staining control (Red).The secondary antibody used was ABflo® 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (AS073) at 1:100.

Overview

Product nameABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
Catalog No.AS073
Host speciesGoat
Purification methodAffinity purification
IsotypeABflo™ 488 conjugated IgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenRabbit IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
Reactivity-
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IF/ICC 1:50 - 1:200
  • FC 1:100 - 1:800
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.025% Sodium Azide, 0.75% BSA, 50% glycerol, pH7.3.
Key applicationImmunofluorescence    Flow Cytometry    
Positive samples
Cellular location
Customer validation

IF(Homo sapiens, Mus musculus, Nicotiana benthamiana)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Immunofluorescence - ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L) (AS073)}

    Immunofluorescence - ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L) (AS073)

    Immunofluorescence analysis of HeLa cells using Alpha-tubulin (ubiquitous) chain Rabbit mAb (AC039) (40x lens), the secondary antibody was ABflo® 488-conjugated AffiniPure Goat Anti antibody (AS073) used at dilution of 1 : 200. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L) (AS073)}

    Immunofluorescence - ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L) (AS073)

    Immunofluorescence analysis of NIH/3T3 cells using Alpha-tubulin (ubiquitous) chain Rabbit mAb (AC039) (40x lens), the secondary antibody was ABflo® 488-conjugated AffiniPure Goat Anti antibody (AS073) used at dilution of 1 : 200. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L) (AS073)}

    Immunofluorescence - ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L) (AS073)

    Immunofluorescence analysis of PC-12 cells using Alpha-tubulin (ubiquitous) chain Rabbit mAb (AC039) (40x lens), the secondary antibody was ABflo® 488-conjugated AffiniPure Goat Anti antibody (AS073) used at dilution of 1 : 200. Blue: DAPI for nuclear staining.
    ABclonal:Flow CytoMetry - ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L) (AS073)}

    Flow CytoMetry - ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L) (AS073)

    Flow cytometric analysis of Positive antibody E-Cadherin Rabbit mAb (2.5μg/mL) in various cells (orange) compare to Rabbit rabbit isotype control (blue) and non-staining control (Red).The secondary antibody used was ABflo® 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (AS073) at 1:100.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS073? Please let us know so that we can cite the reference in this datasheet.