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ABflo® 500 Rabbit IgG isotype control (A25972)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Flow CytoMetry - ABflo® 500 Rabbit IgG isotype control (A25972)

Flow cytometry: 1X10^6 HEL cells (negative control, left) and U266 cells (right) were surface-stained with ABflo® 500 Rabbit anti-Human CD138 (5 μl/Test, orange line) or ABflo® 500 Rabbit IgG isotype control (A25972, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 500 Rabbit IgG isotype control (A25972)

Flow cytometry: 1X10^6 U266 cells were surface-stained with ABflo® 500 Rabbit IgG isotype control (A25972, 5 μl/Test, left) or ABflo® 500 Rabbit anti-Human CD138 (5 μl/Test, right).

Overview

Product nameABflo® 500 Rabbit IgG isotype control
Catalog No.A25972
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC5105-ABf500
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen.An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
ImmunogenA synthesized peptide derived from rabbit IgG isotype control.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular location
    ABclonal:Flow CytoMetry - ABflo® 500 Rabbit IgG isotype control (A25972)}

    Flow CytoMetry - ABflo® 500 Rabbit IgG isotype control (A25972)

    Flow cytometry: 1X10^6 HEL cells (negative control, left) and U266 cells (right) were surface-stained with ABflo® 500 Rabbit anti-Human CD138 (5 μl/Test, orange line) or ABflo® 500 Rabbit IgG isotype control (A25972, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 500 Rabbit IgG isotype control (A25972)}

    Flow CytoMetry - ABflo® 500 Rabbit IgG isotype control (A25972)

    Flow cytometry: 1X10^6 U266 cells were surface-stained with ABflo® 500 Rabbit IgG isotype control (A25972, 5 μl/Test, left) or ABflo® 500 Rabbit anti-Human CD138 (5 μl/Test, right).

    * For research use only. Not for therapeutic or diagnostic purposes.