제품 > 항체 > 단일클론항체(mAb)

ABflo® 594 Rabbit anti-FITC/5-FAM/6-FAM mAb (A24355)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-FITC/5-FAM/6-FAM mAb (A24355)

Flow cytometry:1X10^6 HT-29 cells (negative control, Left) and Raji cells (Right) were surface-stained with Mouse anti-Human CD86 mAb(5 μl/Test, orange line) or ABflo® 594 Mouse IgG1 isotype control (5 μl/Test, blue line), then stained with ABflo® 594 Rabbit anti-FITC/5-FAM/6-FAM mAb(A24355, 5 μl/Test）, Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-FITC/5-FAM/6-FAM mAb (A24355)

Flow cytometry:1X10^6 Raji cells were surface-stained with ABflo® 594 Mouse IgG1 isotype control (5 μl/Test, left) or Mouse anti-Human CD86 mAb(5 μl/Test, right)，then stained with ABflo®594 Rabbit anti-FITC/5-FAM/6-FAM mAb(A24355, 5 μl/Test).

Overview

Product nameABflo® 594 Rabbit anti-FITC/5-FAM/6-FAM mAb
Catalog No.A24355
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgM
CloneNo.ARC51071-IgM-01-ABf594
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
ImmunogenChemical/ Small Molecule corresponding to FITC/5-FAM/6-FAM.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsFITC
Calculated MW
Observed MW
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular location
    ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-FITC/5-FAM/6-FAM mAb (A24355)}

    Flow CytoMetry - ABflo® 594 Rabbit anti-FITC/5-FAM/6-FAM mAb (A24355)

    Flow cytometry:1X10^6 HT-29 cells (negative control, Left) and Raji cells (Right) were surface-stained with Mouse anti-Human CD86 mAb(5 μl/Test, orange line) or ABflo® 594 Mouse IgG1 isotype control (5 μl/Test, blue line), then stained with ABflo® 594 Rabbit anti-FITC/5-FAM/6-FAM mAb(A24355, 5 μl/Test）, Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-FITC/5-FAM/6-FAM mAb (A24355)}

    Flow CytoMetry - ABflo® 594 Rabbit anti-FITC/5-FAM/6-FAM mAb (A24355)

    Flow cytometry:1X10^6 Raji cells were surface-stained with ABflo® 594 Mouse IgG1 isotype control (5 μl/Test, left) or Mouse anti-Human CD86 mAb(5 μl/Test, right)，then stained with ABflo®594 Rabbit anti-FITC/5-FAM/6-FAM mAb(A24355, 5 μl/Test).

    * For research use only. Not for therapeutic or diagnostic purposes.