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ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L) (AS039)

Publications (63) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Immunofluorescence - ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L) (AS039)

Confocal imaging of HeLa cells using [KD Validated] TIMM10 Rabbit mAb (A24996, dilution 1:200) followed by a further incubation with ABflo® 594-conjugated Goat Anti-Rabbit IgG (H+L) (AS039, dilution 1:400). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Flow CytoMetry - ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L) (AS039)

Flow cytometry: 1X10^6 K-562 cells (negative control, left) and A-431 cells (right) were surface-stained with Purified Rabbit anti-Human E-Cadherin mAb(5 μl/Test, orange line) or secondary antibody only (blue line). Non-fluorescently stained K-562 and A-431 cells were used as blank control (red line). ABflo® 594-conjugated Goat Anti-Rabbit IgG (H+L)(AS039, 1:1000) was used as a secondary antibody.

Overview

Product nameABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
Catalog No.AS039
Host speciesGoat
Purification methodAffinity purification
IsotypeHorseradish peroxidase conjugated IgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenRabbit IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IF/ICC 1:50 - 1:200
  • FC 1:100 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 1% BSA, 50% glycerol, pH7.3.
Key applicationImmunofluorescence    Flow Cytometry    
Positive samples
Cellular location
Customer validation

IF(Rattus norvegicus, Equus asinus, Homo sapiens, Mus musculus, Ctenopharyngodon idella, Other, Gallus gallus f. domestica, Anatinae, Ctenopharyngodon idellus, Oryctolagus cuniculus)

IHC(Homo sapiens, Mus musculus, Gallus gallus)

WB(Homo sapiens, Saccharomyces cerevisiae, Mus musculus)

FC(Oryctolagus cuniculus, Mus musculus)

Other(Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Immunofluorescence - ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L) (AS039)}

    Immunofluorescence - ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L) (AS039)

    Confocal imaging of HeLa cells using [KD Validated] TIMM10 Rabbit mAb (A24996, dilution 1:200) followed by a further incubation with ABflo® 594-conjugated Goat Anti-Rabbit IgG (H+L) (AS039, dilution 1:400). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Flow CytoMetry - ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L) (AS039)}

    Flow CytoMetry - ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L) (AS039)

    Flow cytometry: 1X10^6 K-562 cells (negative control, left) and A-431 cells (right) were surface-stained with Purified Rabbit anti-Human E-Cadherin mAb(5 μl/Test, orange line) or secondary antibody only (blue line). Non-fluorescently stained K-562 and A-431 cells were used as blank control (red line). ABflo® 594-conjugated Goat Anti-Rabbit IgG (H+L)(AS039, 1:1000) was used as a secondary antibody.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS039? Please let us know so that we can cite the reference in this datasheet.