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ABflo® 647 Rabbit anti-Goat IgG (Fc) fragment mAb (A22777)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Goat

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Goat IgG (Fc) fragment mAb (A22777)

Flow cytometry:1X10^6 Jurkat cells (negative control, left) and A-431 cells (right) were surface-stained with Goat anti-Human TROP-2 mAb(IgG, κ, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line), followed by ABflo® 647 Rabbit anti-Goat IgG (Fc) fragment mAb(A22777, 5 μl/Test) staining. Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Goat IgG (Fc) fragment mAb (A22777)

Flow cytometry:1X10^6 A-431 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or Goat anti-Human TROP-2 mAb(IgG, κ, right). ABflo® 647 Rabbit anti-Goat IgG (Fc) fragment mAb(A22777, 5 μl/Test)was used as a secondary antibody.

Overview

Product nameABflo® 647 Rabbit anti-Goat IgG (Fc) fragment mAb
Catalog No.A22777
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC56943-ABf647
ImmunogenRecombinant protein of Goat IgG (Fc) fragment.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
ReactivityGoat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Goat IgG (Fc) fragment mAb (A22777)}

    Flow CytoMetry - ABflo® 647 Rabbit anti-Goat IgG (Fc) fragment mAb (A22777)

    Flow cytometry:1X10^6 Jurkat cells (negative control, left) and A-431 cells (right) were surface-stained with Goat anti-Human TROP-2 mAb(IgG, κ, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line), followed by ABflo® 647 Rabbit anti-Goat IgG (Fc) fragment mAb(A22777, 5 μl/Test) staining. Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Goat IgG (Fc) fragment mAb (A22777)}

    Flow CytoMetry - ABflo® 647 Rabbit anti-Goat IgG (Fc) fragment mAb (A22777)

    Flow cytometry:1X10^6 A-431 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or Goat anti-Human TROP-2 mAb(IgG, κ, right). ABflo® 647 Rabbit anti-Goat IgG (Fc) fragment mAb(A22777, 5 μl/Test)was used as a secondary antibody.

    * For research use only. Not for therapeutic or diagnostic purposes.