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ABflo® 647 Rabbit anti-Monkey IgE mAb (A24675)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Cynomolgus monkey

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Monkey IgE mAb (A24675)

Flow cytometry: 1X10^6 HeLa cells (negative control, left) and Monkey PBMC (right) were surface-stained with ABflo® 647 Rabbit anti-Cynomolgus Monkey IgE mAb (ARC61153-01-ABf647, 2 μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Monkey IgE mAb (A24675)

Flow cytometry: 1X10^6 Monkey PBMC were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, left) or ABflo® 647 Rabbit anti-Cynomolgus Monkey IgE mAb (ARC61153-01-ABf647, 2 μg/mL, right).

Overview

Product nameABflo® 647 Rabbit anti-Monkey IgE mAb
Catalog No.A24675
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC61153-ABflo647
IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 99-429 of Monkey IgE(EHH62331.1).
SequenceNKTFGVCSRNFTPPTVKILQSSCDDDGHFPPTIQLLCLISGYTPGAINVTWLENGQVMKVNSPTPPATQEGELASTQSEFTLAQKHWLSDRTYTCQVTYQGTTYNDSTKKCADSNPRGVSAYLSRPSPFDLFISKSPTITCLVVDLAPSKETVNLTWSRASGKPVPHIPATEKKQQRNGTLTVTSILPVVTQDWIEGETYQCRVIHPHLPRALVRSMTKTSGPRAAPEVYVFATPEKLESRDKRTLACLIENFMPEDISVQWLHSDVQLPDTRHSVTQPRKTKGSGFFVFSRLEVTKAEWEQKDEFICRAVHEAASPSWIVQQAVSVNPGK
Gene ID
Swiss Prot
SynonymsIgE; IGHE
Calculated MW
Observed MW
ReactivityCynomolgus monkey
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 0.2μg per 10^6 cells in 100 μl volume
  • Concentration 0.25 mg/mL
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Monkey IgE mAb (A24675)}

    Flow CytoMetry - ABflo® 647 Rabbit anti-Monkey IgE mAb (A24675)

    Flow cytometry: 1X10^6 HeLa cells (negative control, left) and Monkey PBMC (right) were surface-stained with ABflo® 647 Rabbit anti-Cynomolgus Monkey IgE mAb (ARC61153-01-ABf647, 2 μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Monkey IgE mAb (A24675)}

    Flow CytoMetry - ABflo® 647 Rabbit anti-Monkey IgE mAb (A24675)

    Flow cytometry: 1X10^6 Monkey PBMC were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, left) or ABflo® 647 Rabbit anti-Cynomolgus Monkey IgE mAb (ARC61153-01-ABf647, 2 μg/mL, right).

    * For research use only. Not for therapeutic or diagnostic purposes.