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ABflo® 647 Rabbit anti-Mouse IgE mAb (A24275)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse IgE mAb (A24275)

Flow cytometry: 1X10^6 293T cells (negative control, left) and 293T (Transfection, right) cells were intracellularly-stained with ABflo® 647 Rabbit anti-Mouse IgE mAb(A24275, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line).Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse IgE mAb (A24275)

Flow cytometry:1X10^6 293T(Transfection) cells were intracellularly-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Mouse IgE mAb(A24275, 5 μl/Test, right).

Overview

Product nameABflo® 647 Rabbit anti-Mouse IgE mAb
Catalog No.A24275
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC60083-ABflo647
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens 1, 2. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 83-421 of mouse IgE(P06336).
SequenceFNESRTILVRPVNITEPTLELLHSSCDPNAFHSTIQLYCFIYGHILNDVSVSWLMDDREITDTLAQTVLIKEEGKLASTCSKLNITEQQWMSESTFTCKVTSQGVDYLAHTRRCPDHEPRGVITYLIPPSPLDLYQNGAPKLTCLVVDLESEKNVNVTWNQEKKTSVSASQWYTKHHNNATTSITSILPVVAKDWIEGYGYQCIVDHPDFPKPIVRSITKTPGQRSAPEVYVFPPPEEESEDKRTLTCLIQNFFPEDISVQWLGDGKLISNSQHSTTTPLKSNGSNQGFFIFSRLEVAKTLWTQRKQFTCQVIHEALQKPRKLEKTISTSLGNTSLRPS
Gene ID
Swiss Prot
Synonyms IGHE; Ig epsilon chain C region
Calculated MW47kDa
Observed MW
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC (intra) 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application
Positive samples
Cellular locationCell membrane, Secreted

Documents

Certificate of Compliance

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Lot number

    ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse IgE mAb (A24275)}

    Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse IgE mAb (A24275)

    Flow cytometry: 1X10^6 293T cells (negative control, left) and 293T (Transfection, right) cells were intracellularly-stained with ABflo® 647 Rabbit anti-Mouse IgE mAb(A24275, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line).Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse IgE mAb (A24275)}

    Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse IgE mAb (A24275)

    Flow cytometry:1X10^6 293T(Transfection) cells were intracellularly-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Mouse IgE mAb(A24275, 5 μl/Test, right).

    * For research use only. Not for therapeutic or diagnostic purposes.