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ABflo® 647 Rabbit anti-Mouse IgM mAb (A23115)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse IgM mAb (A23115)

Flow cytometry: 1X10^6 NIH/3T3 cells (negative control, left) and C57BL/6 splenocytes (right) were surface-stained with ABflo® 647 Rabbit anti-Mouse IgM mAb (A23115, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse IgM mAb (A23115)

Flow cytometry: 1X10^6 C57BL/6 splenocytes were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Mouse IgM mAb (A23115, 5 μl/Test, right).

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse IgM mAb (A23115)

Flow cytometry: 1X10^6 NIH/3T3 cells (negative control, left) and Mouse PBMC (right) were surface-stained with ABflo® 647 Rabbit anti-Mouse IgM mAb (A23115, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse IgM mAb (A23115)

Flow cytometry: 1X10^6 Mouse PBMC were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Mouse IgM mAb (A23115, 5 μl/Test, right).

Review
ABclonal: review for ABflo® 647 Rabbit anti-Mouse IgM mAb(A23115)

Overview

Product nameABflo® 647 Rabbit anti-Mouse IgM mAb
Catalog No.A23115
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC58894-ABf647
Enables several functions, including antigen binding activity; identical protein binding activity; and immunoglobulin receptor binding activity. Acts upstream of or within several processes, including early endosome to late endosome transport; immunoglobulin mediated immune response; and positive regulation of B cell proliferation. Located in external side of plasma membrane; extracellular space; and perinuclear region of cytoplasm. Part of B cell receptor complex and immunoglobulin complex, circulating. Is expressed in several structures, including brain; hemolymphoid system; and liver. Used to study non-Hodgkin lymphoma and type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in agammaglobulinemia. Orthologous to human IGHM (immunoglobulin heavy constant mu).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 105-454 of mouse IgM (P01872).
SequenceAVAEMNPNVNVFVPPRDGFSGPAPRKSKLICEATNFTPKPITVSWLKDGKLVESGFTTDPVTIENKGSTPQTYKVISTLTISEIDWLNLNVYTCRVDHRGLTFLKNVSSTCAASPSTDILTFTIPPSFADIFLSKSANLTCLVSNLATYETLNISWASQSGEPLETKIKIMESHPNGTFSAKGVASVCVEDWNNRKEFVCTVTHRDLPSPQKKFISKPNEVHKHPPAVYLLPPAREQLNLRESATVTCLVKGFSPADISVQWLQRGQLLPQEKYVTSAPMPEPGAPGFYFTHSILTVTEEEWNSGETYTCVVGHEALPHLVTERTVDKSTGKPTLYNVSLIMSDTGGTCY
Gene ID
Swiss Prot
SynonymsIgm; muH; Igh6; Igh-6; Igh-M
Calculated MW50kDa
Observed MWRefer to figures
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationSecreted, Cell membrane, Single-pass membrane protein.
Customer validation

(Mouse bone marrow)

Documents

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    ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse IgM mAb (A23115)}

    Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse IgM mAb (A23115)

    Flow cytometry: 1X10^6 NIH/3T3 cells (negative control, left) and C57BL/6 splenocytes (right) were surface-stained with ABflo® 647 Rabbit anti-Mouse IgM mAb (A23115, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse IgM mAb (A23115)}

    Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse IgM mAb (A23115)

    Flow cytometry: 1X10^6 C57BL/6 splenocytes were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Mouse IgM mAb (A23115, 5 μl/Test, right).
    ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse IgM mAb (A23115)}

    Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse IgM mAb (A23115)

    Flow cytometry: 1X10^6 NIH/3T3 cells (negative control, left) and Mouse PBMC (right) were surface-stained with ABflo® 647 Rabbit anti-Mouse IgM mAb (A23115, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse IgM mAb (A23115)}

    Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse IgM mAb (A23115)

    Flow cytometry: 1X10^6 Mouse PBMC were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Mouse IgM mAb (A23115, 5 μl/Test, right).

    * For research use only. Not for therapeutic or diagnostic purposes.

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