제품 > 항체 > 다중클론항체(pAb)

ATG8 Rabbit pAb (A22294)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Arabidopsis thaliana, Zea mays, Triticum aestivum

ABclonal:Western blot - ATG8 Rabbit pAb (A22294)

Western blot analysis of lysates from Zea mays, using ATG8 Rabbit pAb (A22294) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameATG8 Rabbit pAb
Catalog No.A22294
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of Triticum aestivum ATG8. (Q7XY24).
SequenceMAKTCFKTEHPLERRQAESARIREKYADRIPVIVEKADKSDVPEIDKKKYLVPADLTVGQFVYVVRKRIKLSPEKAIFVFVNSTLPPTASLMSAIYEENK
Gene ID
Swiss Prot
Synonyms
Calculated MW14kDa
Observed MW15kDa
ReactivityArabidopsis thaliana, Zea mays, Triticum aestivum
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesArabidopsis thaliana, Zea mays, Triticum aestivum
Cellular locationautophagosome.

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    ABclonal:Western blot - ATG8 Rabbit pAb (A22294)}

    Western blot - ATG8 Rabbit pAb (A22294)

    Western blot analysis of lysates from Zea mays, using ATG8 Rabbit pAb (A22294) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.