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Actin(Plant Specific) Rabbit mAb (A23959)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Widely Range, Oryza sativa, Arabidopsis thaliana

ABclonal:Western blot - Actin(Plant Specific) Rabbit mAb (A23959)

Western blot analysis of lysates from Oryza sativa, using Actin(Plant Specific) Rabbit mAb (A23959) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - Actin(Plant Specific) Rabbit mAb (A23959)

Western blot analysis of Arabidopsis thaliana, using Actin(Plant Specific) Rabbit mAb (A23959) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameActin(Plant Specific) Rabbit mAb
Catalog No.A23959
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC62861
Actin is an ancient conserved protein that is encoded by multiple isovariants in multi-cellular organisms. There are eight functional actin genes in the Arabidopsis genome.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of Actin(Plant Specific)(NP_190236.1).
SequenceMADGEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAP
Gene ID
Swiss Prot
SynonymsACT12; Actin(Plant Specific)
Calculated MW 42kDa
Observed MW42kDa
ReactivityWidely Range, Oryza sativa, Arabidopsis thaliana
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:10000 - 1:60000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesOryza sativa, Arabidopsis thaliana
Cellular locationCytoplasm, Cytoskeleton.
Customer validation

WB(Arabidopsis thaliana)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Actin(Plant Specific) Rabbit mAb (A23959)}

    Western blot - Actin(Plant Specific) Rabbit mAb (A23959)

    Western blot analysis of lysates from Oryza sativa, using Actin(Plant Specific) Rabbit mAb (A23959) at 1:10000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - Actin(Plant Specific) Rabbit mAb (A23959)}

    Western blot - Actin(Plant Specific) Rabbit mAb (A23959)

    Western blot analysis of Arabidopsis thaliana, using Actin(Plant Specific) Rabbit mAb (A23959) at 1:10000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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