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Affinity Gel-conjugated Mouse anti HA-Tag mAb (AE059)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Immunoprecipitation - Affinity Gel-conjugated Mouse anti HA-Tag mAb {Anti-HA 亲和凝胶} (AE059)

Immunoprecipitation of SCRN1-HA from 300 µg extracts of 293T cells transfected with a SCRN1 expression vector containing a single N-terminal HA-Tag was performed using 30 µl of Affinity Gel-conjugated Mouse anti HA-Tag mAb (AE059). Affinity Gel-conjugated Mouse Control IgG pAb was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10 % of the total input.Western blot analysis of immunoprecipitates was conducted using Mouse anti HA-Tag mAb (AE065) at a dilution of 1:2000.

Overview

Product nameAffinity Gel-conjugated Mouse anti HA-Tag mAb
Catalog No.AE059
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG1, Kappa
CloneNo.AMC0517
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
ImmunogenA synthetic peptide corresponding to HA tag.
SequenceYPYDVPDYA
Gene ID
Swiss Prot
SynonymsHA; HA tag; HA-tag
Calculated MW
Observed MWRefer to Figures/60kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IP 20μl-40μl Affinity Gel for 100μg-300μg extracts of whole cells
  • Binding Cap 1.5 mg HA protein/mL
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationImmunoprecipitation    
Positive samples293T
Cellular location
Customer validation

Co-IP(Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Immunoprecipitation - Affinity Gel-conjugated Mouse anti HA-Tag mAb {Anti-HA 亲和凝胶} (AE059)}

    Immunoprecipitation - Affinity Gel-conjugated Mouse anti HA-Tag mAb {Anti-HA 亲和凝胶} (AE059)

    Immunoprecipitation of SCRN1-HA from 300 µg extracts of 293T cells transfected with a SCRN1 expression vector containing a single N-terminal HA-Tag was performed using 30 µl of Affinity Gel-conjugated Mouse anti HA-Tag mAb (AE059). Affinity Gel-conjugated Mouse Control IgG pAb was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10 % of the total input.Western blot analysis of immunoprecipitates was conducted using Mouse anti HA-Tag mAb (AE065) at a dilution of 1:2000.

    * For research use only. Not for therapeutic or diagnostic purposes.

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