제품 > 항체 > Tag & 로딩 컨트롤 항체

Affinity Gel-conjugated Mouse anti Myc-Tag mAb (AE060)

Publications (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Immunoprecipitation - Affinity Gel-conjugated Mouse anti Myc-Tag mAb {Anti-Myc 亲和凝胶} (AE060)

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 30 μl Affinity Gel-conjugated Mouse anti Myc-Tag antibody (AE060). Western blot was performed from the immunoprecipitate using Myc-Tag antibody at a dilution of 1:100000.

Overview

Product nameAffinity Gel-conjugated Mouse anti Myc-Tag mAb
Catalog No.AE060
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG1, Kappa
CloneNo.AMC0518
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
ImmunogenA synthetic peptide corresponding to Myc tag.
SequenceEQKLISEEDL
Gene ID
Swiss Prot
SynonymsMyc; Myc tag; Myc-tag
Calculated MW
Observed MW58kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • Binding Cap 0.9 mg Myc protein/mL
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationImmunoprecipitation    
Positive samples
Cellular location
Customer validation

ChIP(Arabidopsis thaliana)

Co-IP(Other)

WB(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Immunoprecipitation - Affinity Gel-conjugated Mouse anti Myc-Tag mAb {Anti-Myc 亲和凝胶} (AE060)}

    Immunoprecipitation - Affinity Gel-conjugated Mouse anti Myc-Tag mAb {Anti-Myc 亲和凝胶} (AE060)

    Immunoprecipitation analysis of 300 μg extracts of 293T cells using 30 μl Affinity Gel-conjugated Mouse anti Myc-Tag antibody (AE060). Western blot was performed from the immunoprecipitate using Myc-Tag antibody at a dilution of 1:100000.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE060? Please let us know so that we can cite the reference in this datasheet.