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Affinity Gel-conjugated Rabbit anti DDDDK-Tag mAb (AE121)

Publication (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Immunoprecipitation - Affinity Gel-conjugated Rabbit anti DDDDK-Tag mAb {Anti-Flag 亲和凝胶} (AE121)

Immunoprecipitation of DDDDK-Tag in 150 µg extracts from 293F cells transfected with DDDDK-Tag using 20 µl Affinity Gel-conjugated Rabbit anti DDDDK-Tag mAb (AE121). Western blot analysis was performed using HRP-conjugated Mouse anti DDDDK-Tag mAb(AE024) at 1:5000 dilution.

Overview

Product nameAffinity Gel-conjugated Rabbit anti DDDDK-Tag mAb
Catalog No.AE121
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.
ImmunogenA synthetic peptide corresponding to DDDDK tag.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsDDDDK; DDDDK tag; DDDDK-tag
Calculated MW
Observed MW40kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IP 20μl-40μl Affinity Gel for 100μg-300μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationImmunoprecipitation    
Positive samples293F
Cellular location
Customer validation

ChIP(Homo sapiens)

WB(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Immunoprecipitation - Affinity Gel-conjugated Rabbit anti DDDDK-Tag mAb {Anti-Flag 亲和凝胶} (AE121)}

    Immunoprecipitation - Affinity Gel-conjugated Rabbit anti DDDDK-Tag mAb {Anti-Flag 亲和凝胶} (AE121)

    Immunoprecipitation of DDDDK-Tag in 150 µg extracts from 293F cells transfected with DDDDK-Tag using 20 µl Affinity Gel-conjugated Rabbit anti DDDDK-Tag mAb (AE121). Western blot analysis was performed using HRP-conjugated Mouse anti DDDDK-Tag mAb(AE024) at 1:5000 dilution.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE121? Please let us know so that we can cite the reference in this datasheet.