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Affinity Gel-conjugated Rabbit anti GFP-Tag mAb (AE119)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Immunoprecipitation - Affinity Gel-conjugated Rabbit anti GFP-Tag mAb {Anti-GFP 亲和凝胶} (AE119)

Immunoprecipitation of GFP-Tag in 150 µg extracts from 293F cells transfected with GFP-Tag using 20 µl Affinity Gel-conjugated Rabbit anti GFP-Tag mAb (AE119). Western blot analysis was performed using HRP-conjugated Mouse anti GFP-Tag mAb (AE030) at 1:5000 dilution.

Overview

Product nameAffinity Gel-conjugated Rabbit anti GFP-Tag mAb
Catalog No.AE119
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. GFP makes for an excellent tool in many forms of biology due to its ability to form internal chromophore without requiring any accessory cofactors, gene products, or enzymes / substrates other than molecular oxygen.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-238 of GFP.
SequenceMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
Gene ID
Swiss Prot
SynonymsGFP; GFP tag; GFP-tag
Calculated MW
Observed MW68kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IP 20μl-40μl Affinity Gel for 100μg-300μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationImmunoprecipitation    
Positive samples293F
Cellular location
Customer validation

Co-IP(Escherichia coli)

IP(Escherichia coli)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Immunoprecipitation - Affinity Gel-conjugated Rabbit anti GFP-Tag mAb {Anti-GFP 亲和凝胶} (AE119)}

    Immunoprecipitation - Affinity Gel-conjugated Rabbit anti GFP-Tag mAb {Anti-GFP 亲和凝胶} (AE119)

    Immunoprecipitation of GFP-Tag in 150 µg extracts from 293F cells transfected with GFP-Tag using 20 µl Affinity Gel-conjugated Rabbit anti GFP-Tag mAb (AE119). Western blot analysis was performed using HRP-conjugated Mouse anti GFP-Tag mAb (AE030) at 1:5000 dilution.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE119? Please let us know so that we can cite the reference in this datasheet.