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Agarose Beads-conjugated Mouse anti V5-Tag mAb (AE029)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

Overview

Product nameAgarose Beads-conjugated Mouse anti V5-Tag mAb
Catalog No.AE029
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG1, Kappa
CloneNo.AMC0514
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
ImmunogenRecombinant protein of V5-Tag.
SequenceGKPIPNPLLGLDST
Gene ID
Swiss Prot
SynonymsV5; V5 tag; V5-tag
Calculated MW
Observed MWRefer to figures
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IP 40ul antibody for 200ug-400ug extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationImmunoprecipitation    
Positive samples
Cellular location

    * For research use only. Not for therapeutic or diagnostic purposes.