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Agarose beads-conjugated anti-HA VHH Single Domain antibody (AE108)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Immunoprecipitation - Agarose beads-conjugated anti-HA VHH Single Domain antibody (AE108)

Immunoprecipitation analysis of 300 ug extract cell lysate from 293T cells transfected with SCRN1 expression vector containing HA and GFP tags with 30μL Anti-HA (Nanobody) Agarose Beads (AE108). Western blot was performed from the immunoprecipitate using Mouse anti HA-Tag mAb (AE065) at a dilition of 1:2000.

Overview

Product nameAgarose beads-conjugated anti-HA VHH Single Domain antibody
Catalog No.AE108
Host speciesAlpaca
Purification methodAffinity purification
IsotypeVHH
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
ImmunogenRecombinant fusion protein of HA.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW53kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IP 30ul antibody (bead slurry) for 200μg-400μg extracts of whole cells
Storage bufferStore at 4℃. Avoid freeze / thaw cycles.
Buffer: 0.03% NaN3, 20% ethanol.
Key applicationImmunoprecipitation    
Positive samples293T
Cellular location

Documents

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    ABclonal:Immunoprecipitation - Agarose beads-conjugated anti-HA VHH Single Domain antibody (AE108)}

    Immunoprecipitation - Agarose beads-conjugated anti-HA VHH Single Domain antibody (AE108)

    Immunoprecipitation analysis of 300 ug extract cell lysate from 293T cells transfected with SCRN1 expression vector containing HA and GFP tags with 30μL Anti-HA (Nanobody) Agarose Beads (AE108). Western blot was performed from the immunoprecipitate using Mouse anti HA-Tag mAb (AE065) at a dilition of 1:2000.

    * For research use only. Not for therapeutic or diagnostic purposes.