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Agarose beads-conjugated anti-mCherry VHH Single Domain antibody (AE073)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Immunoprecipitation - Agarose beads-conjugated anti-mCherry VHH Single Domain antibody (AE073)

Immunoprecipitation analysis of 300 µg extracts from 293T cells transfected with mCherry-tag, using 30µl Agarose beads Anti-mCherry VHH Single Domain antibody (AE073). Western blot analysis was performed using Anti-mCherry-tag antibody (AE171) at 1:5000 dilution.

Overview

Product nameAgarose beads-conjugated anti-mCherry VHH Single Domain antibody
Catalog No.AE073
Host speciesAlpaca
Purification methodAffinity purification
IsotypeVHH
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
ImmunogenRecombinant protein of mCherry.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsmCherry; mCherry tag; mCherry-tag
Calculated MW
Observed MW26kDa/26KD/26KD
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IP 30ul antibody (bead slurry) for 200μg-400μg extracts of whole cells
  • ChIP 500 μL (20 reactions)
  • CoIP 500 μL (20 reactions)
Storage bufferStore at 4℃. Avoid freeze / thaw cycles.
Buffer: 0.03% sodium azide, 20% ethanol
Key applicationImmunoprecipitation    
Positive samples293T transfected with mCherry-tag
Cellular location
Customer validation

Co-IP(Nicotiana benthamiana)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Immunoprecipitation - Agarose beads-conjugated anti-mCherry VHH Single Domain antibody (AE073)}

    Immunoprecipitation - Agarose beads-conjugated anti-mCherry VHH Single Domain antibody (AE073)

    Immunoprecipitation analysis of 300 µg extracts from 293T cells transfected with mCherry-tag, using 30µl Agarose beads Anti-mCherry VHH Single Domain antibody (AE073). Western blot analysis was performed using Anti-mCherry-tag antibody (AE171) at 1:5000 dilution.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE073? Please let us know so that we can cite the reference in this datasheet.