제품 > 항체 > 다중클론항체(pAb)

BPH14 Rabbit pAb (A20668)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - BPH14 Rabbit pAb (A20668)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using BPH14 Rabbit pAb (A20668) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 120s.

Overview

Product nameBPH14 Rabbit pAb
Catalog No.A20668
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
conferring resistance to BPH at seedling and maturity stages of the rice plant
ImmunogenRecombinant protein of Oryza sativa BPH14.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW48kDa
Observed MW100kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesstems, Spikes
Cellular locationcytoplasm
    ABclonal:Western blot - BPH14 Rabbit pAb (A20668)}

    Western blot - BPH14 Rabbit pAb (A20668)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using BPH14 Rabbit pAb (A20668) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 120s.

    * For research use only. Not for therapeutic or diagnostic purposes.