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Biotin Rabbit mAb (A20684)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Other (Wide Range Predicted)

ABclonal:Western blot - Biotin Rabbit mAb (A20684)

Western blot analysis of various lysates, using Biotin Rabbit mAb (A20684) at 1:300 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameBiotin Rabbit mAb
Catalog No.A20684
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG1
CloneNo.ARC50695
Biotin is an organic heterobicyclic compound that consists of 2-oxohexahydro-1H-thieno[3, 4-d]imidazole having a valeric acid substituent attached to the tetrahydrothiophene ring. The parent of the class of biotins. It has a role as a prosthetic group, a coenzyme, a nutraceutical, a human metabolite, a Saccharomyces cerevisiae metabolite, an Escherichia coli metabolite, a mouse metabolite, a cofactor and a fundamental metabolite. It is a member of biotins and a vitamin B7. It is a conjugate acid of a biotinate.
ImmunogenBiotin
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW25-55kDa
ReactivityOther (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesBiotin
Cellular location
Customer validation

IP(Homo sapiens)

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Biotin Rabbit mAb (A20684)}

    Western blot - Biotin Rabbit mAb (A20684)

    Western blot analysis of various lysates, using Biotin Rabbit mAb (A20684) at 1:300 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A20684? Please let us know so that we can cite the reference in this datasheet.