제품 > 항체 > 다중클론항체(pAb)

Biotin Rabbit pAb (A18848)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - Biotin Rabbit pAb (A18848)

Western blot analysis of recombinant Biotin-Goat-Mouse IgG Protein using Biotin Rabbit pAb (A18848) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 100 ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

Overview

Product nameBiotin Rabbit pAb
Catalog No.A18848
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
It is well know now that biotin plays important roles in a variety of critical metabolic reactions in the cell, and thus, is essential for normal human health, growth and development. Biotin is a water-soluble vitamin required by all organisms by virtue of its essential role in carboxylation reactions. Streptavidin and its homologs (together referred to as streptavidin) are widely used in molecular science owing to their highly selective and stable interaction with biotin.
ImmunogenBiotin
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW25kDa(Light chain), 55kDa(Heavy chain)
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesBiotin-Goat-Mouse IgG
Cellular location
Customer validation

IF(Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Biotin Rabbit pAb (A18848)}

    Western blot - Biotin Rabbit pAb (A18848)

    Western blot analysis of recombinant Biotin-Goat-Mouse IgG Protein using Biotin Rabbit pAb (A18848) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 100 ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 45s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A18848? Please let us know so that we can cite the reference in this datasheet.