제품 > 항체 > 다중클론항체(pAb)

CDH8 Rabbit pAb (A18691)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - CDH8 Rabbit pAb (A18691)

Western blot analysis of various lysates using CDH8 Rabbit pAb (A18691) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - CDH8 Rabbit pAb (A18691)

Immunofluorescence analysis of C6 cells using CDH8 Rabbit pAb (A18691) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CDH8 Rabbit pAb (A18691)

Immunofluorescence analysis of NIH/3T3 cells using CDH8 Rabbit pAb (A18691) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCDH8 Rabbit pAb
Catalog No.A18691
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin is expressed in brain and is putatively involved in synaptic adhesion, axon outgrowth and guidance.
ImmunogenRecombinant protein of human CDH8.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW88kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse brain, Rat brain
Cellular location

Documents

Certificate of Compliance

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    ABclonal:Western blot - CDH8 Rabbit pAb (A18691)}

    Western blot - CDH8 Rabbit pAb (A18691)

    Western blot analysis of various lysates using CDH8 Rabbit pAb (A18691) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Immunofluorescence - CDH8 Rabbit pAb (A18691)}

    Immunofluorescence - CDH8 Rabbit pAb (A18691)

    Immunofluorescence analysis of C6 cells using CDH8 Rabbit pAb (A18691) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - CDH8 Rabbit pAb (A18691)}

    Immunofluorescence - CDH8 Rabbit pAb (A18691)

    Immunofluorescence analysis of NIH/3T3 cells using CDH8 Rabbit pAb (A18691) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.