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Cy3-conjugated Goat anti-Mouse IgG (H+L) (AS008)

Publications (51) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Cy3-conjugated Goat anti-Mouse IgG (H+L)
ABclonal:Immunofluorescence - Cy3-conjugated Goat anti-Mouse IgG (H+L) (AS008)

Immunofluorescence analysis of HeLa cells using GAPDH Mouse mAb (AC033, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L)(AS008, dilution 1:200) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.

ABclonal:Immunofluorescence - Cy3-conjugated Goat anti-Mouse IgG (H+L) (AS008)

Immunofluorescence analysis of NIH/3T3 cells using GAPDH Mouse mAb (AC033, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L)(AS008, dilution 1:200) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.

ABclonal:Immunofluorescence - Cy3-conjugated Goat anti-Mouse IgG (H+L) (AS008)

Immunofluorescence analysis of PC-12 cells using GAPDH Mouse mAb (AC033, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L)(AS008, dilution 1:200) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.

ABclonal:Flow CytoMetry - Cy3-conjugated Goat anti-Mouse IgG (H+L) (AS008)

Flow cytometry: 1X10^6 K-562 cells (negative control, left) and Hep G2 cells (right) were surface-stained with Mouse Anti-Human GPC3 mAb (4μg/mL, orange line) or secondary antibody only (blue line). Non-fluorescently stained HepG2 and K-562 cells were used as blank control (red line). Cy3 Goat Anti-Mouse IgG (H+L) (AS008, 1:200) was used as a secondary antibody.

Overview

Product nameCy3-conjugated Goat anti-Mouse IgG (H+L)
Catalog No.AS008
Host speciesGoat
Purification methodAffinity purification
IsotypeCy3 conjugated IgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenMouse IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IF/ICC 1:50 - 1:200
  • FC 1:100 - 1:800
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.025% Sodium Azide, 0.75% BSA, 50% glycerol, pH7.3.
Key applicationImmunofluorescence    Flow Cytometry    
Positive samples
Cellular location
Customer validation

IF(Spodoptera frugiperda, Mus musculus, Rattus norvegicus, Homo sapiens, Pleuronectiformes, Capra hircus, Sus scrofa, Danio rerio, Gallus gallus)

WB(Mus musculus)

FC(Danio rerio, Homo sapiens)

IHC(Homo sapiens, Mus musculus, Misgurnus anguillicaudatus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Immunofluorescence - Cy3-conjugated Goat anti-Mouse IgG (H+L) (AS008)}

    Immunofluorescence - Cy3-conjugated Goat anti-Mouse IgG (H+L) (AS008)

    Immunofluorescence analysis of HeLa cells using GAPDH Mouse mAb (AC033, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L)(AS008, dilution 1:200) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.
    ABclonal:Immunofluorescence - Cy3-conjugated Goat anti-Mouse IgG (H+L) (AS008)}

    Immunofluorescence - Cy3-conjugated Goat anti-Mouse IgG (H+L) (AS008)

    Immunofluorescence analysis of NIH/3T3 cells using GAPDH Mouse mAb (AC033, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L)(AS008, dilution 1:200) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.
    ABclonal:Immunofluorescence - Cy3-conjugated Goat anti-Mouse IgG (H+L) (AS008)}

    Immunofluorescence - Cy3-conjugated Goat anti-Mouse IgG (H+L) (AS008)

    Immunofluorescence analysis of PC-12 cells using GAPDH Mouse mAb (AC033, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L)(AS008, dilution 1:200) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.
    ABclonal:Flow CytoMetry - Cy3-conjugated Goat anti-Mouse IgG (H+L) (AS008)}

    Flow CytoMetry - Cy3-conjugated Goat anti-Mouse IgG (H+L) (AS008)

    Flow cytometry: 1X10^6 K-562 cells (negative control, left) and Hep G2 cells (right) were surface-stained with Mouse Anti-Human GPC3 mAb (4μg/mL, orange line) or secondary antibody only (blue line). Non-fluorescently stained HepG2 and K-562 cells were used as blank control (red line). Cy3 Goat Anti-Mouse IgG (H+L) (AS008, 1:200) was used as a secondary antibody.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS008? Please let us know so that we can cite the reference in this datasheet.