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DiMethyl-eEF1A-K55 Rabbit pAb (A17987)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - DiMethyl-eEF1A-K55 Rabbit pAb (A17987)

Western blot analysis of lysates from 293T cells, using DiMethyl-eEF1A-K55 Rabbit pAb (A17987) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameDiMethyl-eEF1A-K55 Rabbit pAb
Catalog No.A17987
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenA synthetic dimethylated peptide around K55 of human eEF1A (NP_001393.1).
SequenceSFKYA
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW50kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - DiMethyl-eEF1A-K55 Rabbit pAb (A17987)}

    Western blot - DiMethyl-eEF1A-K55 Rabbit pAb (A17987)

    Western blot analysis of lysates from 293T cells, using DiMethyl-eEF1A-K55 Rabbit pAb (A17987) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.