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EV71 3C Rabbit mAb (A23772)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Enterovirus

ABclonal:Western blot - EV71 3C Rabbit mAb (A23772)

Western blot analysis lysates of recombinant EV71 3C Protein, using EV71 3C Rabbit mAb (A23772) at 1:160000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 5ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

Overview

Product nameEV71 3C Rabbit mAb
Catalog No.A23772
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC59088
ImmunogenRecombinant protein of enterovirus EV71 3C.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW20KDa
Observed MW40kDa
ReactivityEnterovirus
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:10000 - 1:170000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRecombinant protein of enterovirus EV71 3C
Cellular location
Customer validation

WB(Homo sapiens,Sus scrofa)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - EV71 3C Rabbit mAb (A23772)}

    Western blot - EV71 3C Rabbit mAb (A23772)

    Western blot analysis lysates of recombinant EV71 3C Protein, using EV71 3C Rabbit mAb (A23772) at 1:160000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 5ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 20s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A23772? Please let us know so that we can cite the reference in this datasheet.