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EV71 3C Rabbit pAb (A20687)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Other (Wide Range Predicted)

ABclonal:Western blot - EV71 3C Rabbit pAb (A20687)

Western blot analysis of lysates from HeLa cells, using EV71 3C Rabbit pAb (A20687) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameEV71 3C Rabbit pAb
Catalog No.A20687
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enterovirus 71 (EV71) is the main pathogen of the hand, foot, and mouth disease. It was firstly isolated from sputum specimens of infants with central nervous system diseases in California in 1969, and has been repeatedly reported in various parts of the world, especially in the Asia-Pacific region. Infection with EV71 can lead tosevere neurological complications such as encephalitis, acuteflaccid paralysis, and pulmonary edema in children (1). EV71 belongs to the genus Enterovirus within the Picorna viridae family, which is a non-enveloped virus with a singlepositive-stranded RNA genome.EV71 3C protein is a 183 amino acid cysteine protease that can cleave most structural and non-structural proteins of EV71. Based on the analysis and understanding of EV71 3C protease, it is helpful to study and treat diseases caused by EV71 virus infection. The EV71 3C protease promotes virus replication by cleaving EV71 synthesis or host proteins. Moreover, EV71 3C protease inhibits the innate immune system and causes apoptosis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human EV71 3C. (B9VUU3).
SequenceMGSQVSTQRSGSHENSNSATEGSTINYTTINYYKDSYAATAGKQSLKQDPDKFANPVKDIFTEMAAPLKSPSAEACGYSDRVAQLTIGNSTITTQEAANIIVGYGEWPSYCSDSDATAVDKPTRPDVSVNRFYTLDTKLWEKSSKGWYWKFPDVLTETGVFGQNAQFHYLYRSGFCIHVQCNA
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW20kDa
ReactivityOther (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa
Cellular location
Customer validation

WB(Enterovirus 71, Other)

Documents

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    ABclonal:Western blot - EV71 3C Rabbit pAb (A20687)}

    Western blot - EV71 3C Rabbit pAb (A20687)

    Western blot analysis of lysates from HeLa cells, using EV71 3C Rabbit pAb (A20687) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A20687? Please let us know so that we can cite the reference in this datasheet.