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EV71 3D Rabbit mAb (A22651)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - EV71 3D Rabbit mAb (A22651)

Western blot analysis of lysates from wild type (WT) and HeLa cells transfected with EV71 3D using EV71 3D Rabbit mAb (A22651) at 1:20000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameEV71 3D Rabbit mAb
Catalog No.A22651
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC58400
ImmunogenRecombinant protein of Enterovirus EV71 3D.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW51kDa
Observed MW60kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:20000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa transfected with EV71 3D
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - EV71 3D Rabbit mAb (A22651)}

    Western blot - EV71 3D Rabbit mAb (A22651)

    Western blot analysis of lysates from wild type (WT) and HeLa cells transfected with EV71 3D using EV71 3D Rabbit mAb (A22651) at 1:20000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.

    * For research use only. Not for therapeutic or diagnostic purposes.