제품 > 항체 > 단일클론항체(mAb)

FITC/5-FAM/6-FAM Rabbit mAb (A20797)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal: - FITC/5-FAM/6-FAM Rabbit mAb (A20797)

Immobilized biotin-6 Fam, biotin-5 Fam, biotin-FITC, at 1 μg/mL (25 μl/well), of which EC50 ranges are 0.27-0.54 ng/mL, 0.18-0.31 ng/mL and 0.12-0.39 ng/mL , can bind anti 6-Fam/ 5-Fam/ FITC mAb (A20797), respectively.

ABclonal:Western blot - FITC/5-FAM/6-FAM Rabbit mAb (A20797)

Westemn blot analysis of recombiant FITC conjugated goat IG protein using FITC/5-FAM/6-FAM Rabbit mAb (A20797) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameFITC/5-FAM/6-FAM Rabbit mAb
Catalog No.A20797
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC51071
Fluorescein isothiocyanate (FITC) labeling is a common technique with a wide range of? applications because it reacts quickly with amines and due to its high quantum efficacy. Due to its high molecular absorptivity, using FITC labels is preferred over conventional colorimetric labels and radio labels because fluorophores like FITC are bright, easier to work with, and don’t require special waste handling. Proteins, substrates, peptide hormones, and antibodies? labeled by FITC can be used as probes in flow cytometry, enzyme kinetics, and immunocytochemistry, as well as in the detection of receptors on the surface of? the target cells.5-FAM is the purified single isomer of carboxyfluorescein. It is one of the most popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. It has been predominantly used to develop a variety of green fluorescent peptides that can be excited with the 488 nm line of the Ar laser. It has also been used to prepare various small fluorescent molecules.6-FAM (6-Carboxyfluorescein) contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility, fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser.
ImmunogenFITC
SequenceFITC conjugated to KLH
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW25kDa/55kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:12000
  • ELISA 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples
Cellular location

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal: - FITC/5-FAM/6-FAM Rabbit mAb (A20797)}

    - FITC/5-FAM/6-FAM Rabbit mAb (A20797)

    Immobilized biotin-6 Fam, biotin-5 Fam, biotin-FITC, at 1 μg/mL (25 μl/well), of which EC50 ranges are 0.27-0.54 ng/mL, 0.18-0.31 ng/mL and 0.12-0.39 ng/mL , can bind anti 6-Fam/ 5-Fam/ FITC mAb (A20797), respectively.
    ABclonal:Western blot - FITC/5-FAM/6-FAM Rabbit mAb (A20797)}

    Western blot - FITC/5-FAM/6-FAM Rabbit mAb (A20797)

    Westemn blot analysis of recombiant FITC conjugated goat IG protein using FITC/5-FAM/6-FAM Rabbit mAb (A20797) at 1:2000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.

    * For research use only. Not for therapeutic or diagnostic purposes.