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FITC Anti-Mouse CD3e mAb (A23322)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Flow CytoMetry - FITC Anti-Mouse CD3e mAb (A23322)

Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes were surface-stained with FITC Armenian Hamster IgG isotype control (5 μl/Test, left) or FITC Anti-Mouse CD3e mAb (A23322, 2 μg/mL, right).

Overview

Product nameFITC Anti-Mouse CD3e mAb
Catalog No.A23322
Host speciesArmenian Hamster
Purification methodAffinity purification
IsotypeArmenian Hamster IgG
CloneNo.ACC0001
Predicted to enable several functions, including SH3 domain binding activity; identical protein binding activity; and protein heterodimerization activity. Involved in nervous system development and positive regulation of cell adhesion. Acts upstream of or within several processes, including positive regulation of T cell activation; positive regulation of cytokine production; and regulation of signal transduction. Located in several cellular components, including dendritic spine; external side of plasma membrane; and immunological synapse. Part of alpha-beta T cell receptor complex. Is expressed in colon and hemolymphoid system. Human ortholog(s) of this gene implicated in immunodeficiency 18. Orthologous to human CD3E (CD3e molecule).
ImmunogenRecombinant protein of Mouse CD3e.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsCD3; T3e; CD3epsilon
Calculated MW
Observed MWRefer to figures
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC The suggested use of this reagent is ≤0.5 µg per 10^6 cells in 100 µl volume.
Storage bufferStore at 4℃. Avoid freeze / thaw cycles.
Buffer: 10 mM NaH₂PO₄, 150 mM NaCl, 0.09% NaN₃, 0.1% gelatin, pH7.2
Key applicationFlow Cytometry    
Positive samples
Cellular location
    ABclonal:Flow CytoMetry - FITC Anti-Mouse CD3e mAb (A23322)}

    Flow CytoMetry - FITC Anti-Mouse CD3e mAb (A23322)

    Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes were surface-stained with FITC Armenian Hamster IgG isotype control (5 μl/Test, left) or FITC Anti-Mouse CD3e mAb (A23322, 2 μg/mL, right).

    * For research use only. Not for therapeutic or diagnostic purposes.