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FITC-conjugated Donkey anti-Rabbit IgG (H+L) (AS042)

Publications (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Immunofluorescence - FITC-conjugated Donkey anti-Rabbit IgG (H+L) (AS042)

Immunofluorescence analysis of PC-12 cells using Vimentin Rabbit mAb (A19607) at a dilution of 1:100 (40x lens). Secondary antibody: FITC Donkey Anti-Rabbit IgG (H+L) (AS042) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - FITC-conjugated Donkey anti-Rabbit IgG (H+L) (AS042)

Immunofluorescence analysis of HeLa cells using Vimentin Rabbit mAb (A19607) at a dilution of 1:100 (40x lens). Secondary antibody: FITC Donkey Anti-Rabbit IgG (H+L) (AS042) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Flow CytoMetry - FITC-conjugated Donkey anti-Rabbit IgG (H+L) (AS042)

Flow cytometric analysis of Positive antibody E-Cadherin Rabbit mAb (2.5μg/mL) in various cells (orange) compare to Rabbit rabbit isotype control (blue) and non-staining control (Red).The secondary antibody used was FITC Donkey Anti-Rabbit IgG (H+L) (AS042) at 1:100.

Overview

Product nameFITC-conjugated Donkey anti-Rabbit IgG (H+L)
Catalog No.AS042
Host speciesDonkey
Purification methodAffinity purification
IsotypeFluorescein conjugated IgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenRabbit IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IF/ICC 1:50 - 1:200
  • FC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.025% Sodium Azide, 0.75% BSA, 50% glycerol, pH7.3.
Key applicationImmunofluorescence    Flow Cytometry    
Positive samples
Cellular location
Customer validation

FC(Mus musculus)

IF(Oryctolagus cuniculus, Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Immunofluorescence - FITC-conjugated Donkey anti-Rabbit IgG (H+L) (AS042)}

    Immunofluorescence - FITC-conjugated Donkey anti-Rabbit IgG (H+L) (AS042)

    Immunofluorescence analysis of PC-12 cells using Vimentin Rabbit mAb (A19607) at a dilution of 1:100 (40x lens). Secondary antibody: FITC Donkey Anti-Rabbit IgG (H+L) (AS042) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - FITC-conjugated Donkey anti-Rabbit IgG (H+L) (AS042)}

    Immunofluorescence - FITC-conjugated Donkey anti-Rabbit IgG (H+L) (AS042)

    Immunofluorescence analysis of HeLa cells using Vimentin Rabbit mAb (A19607) at a dilution of 1:100 (40x lens). Secondary antibody: FITC Donkey Anti-Rabbit IgG (H+L) (AS042) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Flow CytoMetry - FITC-conjugated Donkey anti-Rabbit IgG (H+L) (AS042)}

    Flow CytoMetry - FITC-conjugated Donkey anti-Rabbit IgG (H+L) (AS042)

    Flow cytometric analysis of Positive antibody E-Cadherin Rabbit mAb (2.5μg/mL) in various cells (orange) compare to Rabbit rabbit isotype control (blue) and non-staining control (Red).The secondary antibody used was FITC Donkey Anti-Rabbit IgG (H+L) (AS042) at 1:100.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS042? Please let us know so that we can cite the reference in this datasheet.