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Fluorescein Isothiocyanate/FITC Rabbit pAb (A20385)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - Fluorescein Isothiocyanate/FITC Rabbit pAb (A20385)

The FITC rabbit polyclonal antibody (A20385) are tested in Western blot against FITC conjugated Goat IgG, TRITC conjugated Goat IgG and unconjugated Goat IgG
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: Refer to figures.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameFluorescein Isothiocyanate/FITC Rabbit pAb
Catalog No.A20385
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. FITC is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is also widely used as a hapten to label different proteins.
ImmunogenFITC
SequenceFITC conjugated to KLH
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW25kDa/55kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples
Cellular location
Customer validation

IF(Gallus gallus, Homo sapiens)

WB(Mus musculus)

Documents

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    ABclonal:Western blot - Fluorescein Isothiocyanate/FITC Rabbit pAb (A20385)}

    Western blot - Fluorescein Isothiocyanate/FITC Rabbit pAb (A20385)

    The FITC rabbit polyclonal antibody (A20385) are tested in Western blot against FITC conjugated Goat IgG, TRITC conjugated Goat IgG and unconjugated Goat IgG
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: Refer to figures.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A20385? Please let us know so that we can cite the reference in this datasheet.