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Goat Anti-Human IgG (H+L) (AS084)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Goat Anti-Human IgG (H+L) (AS084)

Western blot analysis of lysates from Human plasma, using Goat Anti-Human IgG (H+L) (AS084 ) at 1:1000 dilution.
Secondary antibody: HRP Donkey Anti-Goat IgG (H+L) (AS031) at 1:4000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - Goat Anti-Human IgG (H+L) (AS084)

Western blot analysis of lysates from Human IgG using Goat Anti-Human IgG (H+L) (AS002) at 1:5000 dilution.
Lysates/proteins: 5ng - 10ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameGoat Anti-Human IgG (H+L)
Catalog No.AS084
Host speciesGoat
Purification methodAffinity purification
IsotypeIgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenHuman IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW25kDa(Light chain), 55kDa(Heavy chain)
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHuman IgG1, Human IgG2, Human IgG4
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Goat Anti-Human IgG (H+L) (AS084)}

    Western blot - Goat Anti-Human IgG (H+L) (AS084)

    Western blot analysis of lysates from Human plasma, using Goat Anti-Human IgG (H+L) (AS084 ) at 1:1000 dilution.
    Secondary antibody: HRP Donkey Anti-Goat IgG (H+L) (AS031) at 1:4000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - Goat Anti-Human IgG (H+L) (AS084)}

    Western blot - Goat Anti-Human IgG (H+L) (AS084)

    Western blot analysis of lysates from Human IgG using Goat Anti-Human IgG (H+L) (AS002) at 1:5000 dilution.
    Lysates/proteins: 5ng - 10ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.