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Goat Anti-Rabbit IgG(H+L) (AS070)

Publications (12) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal: - Goat Anti-Rabbit IgG(H+L) (AS070)

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K-562 cells with 1 μg of TriMethyl-Histone H3-K4 Rabbit mAb (A22146), followed by incubation with Goat Anti-Rabbit IgG(H+L)(AS070). The CUT&Tag results denote the enrichment pattern of TriMethyl-Histone H3-K4 around RPL30 gene.

ABclonal: - Goat Anti-Rabbit IgG(H+L) (AS070)

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K-562 cells with 1 μg of TriMethyl-Histone H3-K4 Rabbit mAb (A22146), followed by incubation with Goat Anti-Rabbit IgG (H+L)(AS070). The CUT&Tag results denote the enrichment pattern of TriMethyl-Histone H3-K4 across chromosome 8 (upper panel) and the genomic region encompassing RPL30, a representative gene enriched in TriMethyl-Histone H3-K4 (lower panel).

ABclonal:Western blot - Goat Anti-Rabbit IgG(H+L) (AS070)

Western blot analysis of recombinant Rabbit/Mouse/Rat control IgG Protein using Goat Anti-Rabbit IgG(H+L) (AS070) at 1:1000 dilution.
Secondary antibody: HRP Donkey Anti-Goat IgG (H+L) (AS031) at 1:10000 dilution.
Lysates/proteins: 100ng/50ng/25ng/10ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

Overview

Product nameGoat Anti-Rabbit IgG(H+L)
Catalog No.AS070
Host speciesGoat
Purification methodAffinity purification
IsotypeIgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenRabbit IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW25kDa(Light chain), 55kDa(Heavy chain)/
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • CUT&Tag 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRabbit control IgG
Cellular location
Customer validation

WB(Homo sapiens, Mus musculus, Rattus norvegicus)

CUT&Tag(Anatinae, Mus musculus, Homo sapiens)

RIP(Homo sapiens)

IHC(Mus musculus)

IF(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal: - Goat Anti-Rabbit IgG(H+L) (AS070)}

    - Goat Anti-Rabbit IgG(H+L) (AS070)

    CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K-562 cells with 1 μg of TriMethyl-Histone H3-K4 Rabbit mAb (A22146), followed by incubation with Goat Anti-Rabbit IgG(H+L)(AS070). The CUT&Tag results denote the enrichment pattern of TriMethyl-Histone H3-K4 around RPL30 gene.
    ABclonal: - Goat Anti-Rabbit IgG(H+L) (AS070)}

    - Goat Anti-Rabbit IgG(H+L) (AS070)

    CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K-562 cells with 1 μg of TriMethyl-Histone H3-K4 Rabbit mAb (A22146), followed by incubation with Goat Anti-Rabbit IgG (H+L)(AS070). The CUT&Tag results denote the enrichment pattern of TriMethyl-Histone H3-K4 across chromosome 8 (upper panel) and the genomic region encompassing RPL30, a representative gene enriched in TriMethyl-Histone H3-K4 (lower panel).
    ABclonal:Western blot - Goat Anti-Rabbit IgG(H+L) (AS070)}

    Western blot - Goat Anti-Rabbit IgG(H+L) (AS070)

    Western blot analysis of recombinant Rabbit/Mouse/Rat control IgG Protein using Goat Anti-Rabbit IgG(H+L) (AS070) at 1:1000 dilution.
    Secondary antibody: HRP Donkey Anti-Goat IgG (H+L) (AS031) at 1:10000 dilution.
    Lysates/proteins: 100ng/50ng/25ng/10ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS070? Please let us know so that we can cite the reference in this datasheet.